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Plasmonic Patch Boosts Diagnostic Fluorescence Intensity

By MedImaging International staff writers
Posted on 18 Jul 2018

Image: A high-tech patch utilizes metal nanostructures to increase the fluorescence intensity by 100 times (Photo courtesy of WUSTL).

A new study shows how a high-tech add patch uses metal nanostructures to increase the fluorescent brightness of diagnostic tests one-hundred fold.

Developed by researchers at Washington University School of Medicine (WUSTL; St. Louis, MO, USA) and the Air Force Research Laboratory (AFRL; Wright-Patterson Air Force Base, OH, USA), the plasmonic patch is a flexible, conformal elastomeric film with adsorbed nanostructures that offer a simple and universal method to provide large (up to 100-fold) and uniform fluorescence enhancement on a variety of surfaces, by simply transferring the patch to the surface in question.

According to the researchers, the plasmonic patch can improve the sensitivity and limit of detection of fluorescence-based immunoassays, and represents a fundamental enabling technology to immediately improve the sensitivity of existing disease, biomarker, and application agnostic analytic methodologies. The researchers suggest it could be particularly useful in a microarray chip, which enables simultaneous detection of tens to hundreds of analytes in a single experiment. The study describing the plasmonic patch was published in the July 2018 issue of Light: Science and Applications.

"It's a thin layer of elastic, transparent material with gold nanorods or other plasmonic nanostructures absorbed on the top,” said lead author Jingyi Luan, MSc, a graduate student at WUSTL. “These nanostructures act as antennae; they concentrate light into a tiny volume around the molecules emitting fluorescence. The fluorescence is dramatic, making it easier to visualize. The patch can be imagined to be a magnifying glass for the light.”

“The plasmonic patch will enable the detection of low abundance analytes in combination with conventional detection methodologies, which is the beauty of our approach,” concluded co-senior author Rajesh Naik, PhD, chief scientist of AFRL's 711th Human Performance Wing. “All a researcher or lab tech needs to do is prepare the sample in the usual method, apply the patch over the top, and then scan the sample as usual.”

Fluorescence-based techniques have radically transformed biology and life sciences by unraveling the genomic, transcriptomic, and proteomic signatures of disease development, progression, and response to therapy. However, the occurrence of a “feeble signal” has been a persistent and recurring problem in imaging techniques that rely on fluorescence. Overcoming this fundamental challenge without the use of specialized reagents, equipment, or significant modifications to well-established procedures is a holy grail in the field of biomedical optics.

Related Links:
Washington University School of Medicine
Air Force Research Laboratory

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69 (96.08268737793): select jos_facebook.link from jos_facebook where cat=10

70 (50.067901611328): select jos_google_plus.link from jos_google_plus where cat=10

71 (41.961669921875): select jos_linkedin.link from jos_linkedin where cat=10

72 (735.04447937012): SELECT fid FROM jos_deeppockets WHERE id=294774190 AND published='1' union SELECT fid FROM jos_deeppockets_archive WHERE id=294774190 AND published='1' LIMIT 1

73 (66.041946411133): SELECT id,name,sponsor_text FROM jos_deeppockets dp left outer join jos_channel_sponsor cs on dp.id=cs.fid WHERE cat=10 AND TYPE='file_folder' AND published=1 AND id=215 ORDER BY `ordering`

74 (925.06408691406): SELECT fid FROM jos_deeppockets WHERE cat=10 AND id=294774190 AND (type='deeppockets_item' OR type='deeppockets_item_link') AND published='1' LIMIT 1

75 (434.87548828125): SELECT fid FROM jos_deeppockets_archive WHERE cat=10 AND id=294774190 AND (type='deeppockets_item' OR type='deeppockets_item_link') AND published='1' LIMIT 1

76 (75.101852416992): SELECT id,name,title FROM jos_deeppockets WHERE cat=10 AND TYPE='file_folder' AND published=1 AND id != 294743300 AND id != 294743301 AND id != 294743302 AND id != 294743303 AND id != 294743304 AND id != 294743305 AND id != 294743306 AND id != 294743307 AND id != 294743308 AND id != 294743309 AND id != 294743310 AND metakey != 'aacc' ORDER BY `ordering`

77 (!!!!! 2551.794052124): SELECT show_live FROM jos_com_show_setting LIMIT 1

78 (!!!!! 6892.9195404053): SELECT show_live FROM jos_com_show_setting LIMIT 1

79 (!!!!! 2312.183380127): SELECT show_live FROM jos_com_show_setting LIMIT 1

80 (!!!!! 4783.8687896729): SELECT show_live FROM jos_com_show_setting LIMIT 1

81 (!!!!! 1760.0059509277): SELECT show_live FROM jos_com_show_setting LIMIT 1

82 (206.94732666016): SELECT show_live FROM jos_com_show_setting LIMIT 1

83 (292.06275939941): SELECT show_live FROM jos_com_show_setting LIMIT 1

84 (55.074691772461): SELECT show_live FROM jos_com_show_setting LIMIT 1

85 (191.21170043945): SELECT show_live FROM jos_com_show_setting LIMIT 1

86 (493.04962158203): SELECT show_live FROM jos_com_show_setting LIMIT 1

87 (95.844268798828): SELECT show_live FROM jos_com_show_setting LIMIT 1

88 (51.975250244141): SELECT show_live FROM jos_com_show_setting LIMIT 1

89 (41.961669921875): SELECT show_live FROM jos_com_show_setting LIMIT 1

90 (38.862228393555): SELECT show_live FROM jos_com_show_setting LIMIT 1

91 (!!!!! 1085.9966278076): SELECT fid FROM jos_deeppockets WHERE cat=10 AND id=294774190 AND (type='deeppockets_item' OR type='deeppockets_item_link') AND published='1' LIMIT 1

92 (57.93571472168): SELECT fid FROM jos_deeppockets_archive WHERE cat=10 AND id=294774190 AND (type='deeppockets_item' OR type='deeppockets_item_link') AND published='1' LIMIT 1

93 (51.021575927734): SELECT id,name,title FROM jos_deeppockets WHERE cat=10 AND TYPE='file_folder' AND published=1 AND id != 294743300 AND id != 294743301 AND id != 294743302 AND id != 294743303 AND id != 294743304 AND id != 294743305 AND id != 294743306 AND id != 294743307 AND id != 294743308 AND id != 294743309 AND id != 294743310 AND metakey != 'aacc' ORDER BY `ordering`

94 (98.943710327148): SELECT show_live FROM jos_com_show_setting LIMIT 1

95 (46.968460083008): SELECT show_live FROM jos_com_show_setting LIMIT 1

96 (905.99060058594): SELECT show_live FROM jos_com_show_setting LIMIT 1

97 (81.062316894531): SELECT show_live FROM jos_com_show_setting LIMIT 1

98 (49.114227294922): SELECT show_live FROM jos_com_show_setting LIMIT 1

99 (41.007995605469): SELECT show_live FROM jos_com_show_setting LIMIT 1

100 (51.975250244141): SELECT show_live FROM jos_com_show_setting LIMIT 1

101 (40.054321289062): SELECT show_live FROM jos_com_show_setting LIMIT 1

102 (39.100646972656): SELECT show_live FROM jos_com_show_setting LIMIT 1

103 (!!!!! 3458.9767456055): SELECT show_live FROM jos_com_show_setting LIMIT 1

104 (122.0703125): SELECT show_live FROM jos_com_show_setting LIMIT 1

105 (147.10426330566): SELECT show_live FROM jos_com_show_setting LIMIT 1

106 (53.882598876953): SELECT show_live FROM jos_com_show_setting LIMIT 1

107 (!!!!! 3476.1428833008): SELECT show_live FROM jos_com_show_setting LIMIT 1

108 (180.00602722168): SELECT * FROM jos_deeppockets WHERE 1=1 AND id=294774190 AND published='1' union SELECT * FROM jos_deeppockets_archive WHERE 1=1 AND id=294774190 AND published='1' LIMIT 1

109 (78.916549682617): SELECT * FROM jos_deeppockets WHERE 1=1 AND id=294774190 AND published='1' union SELECT * FROM jos_deeppockets_archive WHERE 1=1 AND id=294774190 AND published='1' LIMIT 1

110 (114.20249938965): SELECT dp.*, ch.title as channel, exp.name as author_name, exp.specialization as author_specialization, exp.images as author_photo FROM jos_deeppockets as dp LEFT JOIN jos_experts as exp ON exp.id = dp.author LEFT JOIN jos_deeppockets as ch ON dp.fid = ch.id WHERE 1=1 AND dp.cat=10 AND dp.id=294774190 AND (dp.type='deeppockets_item' OR dp.type='deeppockets_item_link') AND dp.published='1' LIMIT 1

111 (761.03210449219): SELECT dp.*, ch.title as channel, exp.name as author_name, exp.specialization as author_specialization, exp.images as author_photo FROM jos_deeppockets_archive as dp LEFT JOIN jos_experts as exp ON exp.id = dp.author LEFT JOIN jos_deeppockets as ch ON dp.fid = ch.id WHERE 1=1 AND dp.cat=10 AND dp.id=294774190 AND (dp.type='deeppockets_item' OR dp.type='deeppockets_item_link') AND dp.published='1' LIMIT 1

112 (!!!!! 2619.9817657471): SELECT * FROM jos_deeppockets WHERE 1=1 AND id=294774190 AND published='1' union SELECT * FROM jos_deeppockets_archive WHERE 1=1 AND id=294774190 AND published='1' LIMIT 1

113 (167.1314239502): SELECT * FROM jos_deeppockets WHERE 1=1 AND id=294774190 AND published='1' union SELECT * FROM jos_deeppockets_archive WHERE 1=1 AND id=294774190 AND published='1' LIMIT 1

114 (96.08268737793): SELECT dp.*, ch.title as channel, exp.name as author_name, exp.specialization as author_specialization, exp.images as author_photo FROM jos_deeppockets as dp LEFT JOIN jos_experts as exp ON exp.id = dp.author LEFT JOIN jos_deeppockets as ch ON dp.fid = ch.id WHERE 1=1 AND dp.id=294774190 AND (dp.type='deeppockets_item' OR dp.type='deeppockets_item_link') AND dp.published='1' LIMIT 1

115 (!!!!! 2766.8476104736): SELECT dp.*, ch.title as channel, exp.name as author_name, exp.specialization as author_specialization, exp.images as author_photo FROM jos_deeppockets_archive as dp LEFT JOIN jos_experts as exp ON exp.id = dp.author LEFT JOIN jos_deeppockets as ch ON dp.fid = ch.id WHERE 1=1 AND dp.id=294774190 AND (dp.type='deeppockets_item' OR dp.type='deeppockets_item_link') AND dp.published='1' LIMIT 1

116 (!!!!! 5280.0178527832): SELECT dp.name, dp.id, dp2.name as channel, ex.name as author_name, ex.specialization, ex.images FROM jos_deeppockets as dp LEFT JOIN jos_experts as ex ON ex.id = dp.author LEFT JOIN jos_deeppockets as dp2 ON dp2.id = dp.fid WHERE dp.author != 0 AND dp.cat = 10 AND dp.published = 1 AND ( (dp.fid = 294743300) OR (dp.fid = 294743301) OR (dp.fid = 294743302) OR (dp.fid = 294743303) OR (dp.fid = 294743304) OR (dp.fid = 294743305) OR (dp.fid = 294743306) OR (dp.fid = 294743307) OR (dp.fid = 294743308) OR (dp.fid = 294743309) OR (dp.fid = 294743310) ) ORDER BY RAND() LIMIT 1

117 (!!!!! 1088.1423950195): SELECT dp.name, dp.id, dp2.name as channel, ex.name as author_name, ex.specialization, ex.images FROM jos_deeppockets as dp LEFT JOIN jos_experts as ex ON ex.id = dp.author LEFT JOIN jos_deeppockets as dp2 ON dp2.id = dp.fid WHERE dp.author != 0 AND dp.cat = 10 AND dp.published = 1 AND ( (dp.fid = 294743300) OR (dp.fid = 294743301) OR (dp.fid = 294743302) OR (dp.fid = 294743303) OR (dp.fid = 294743304) OR (dp.fid = 294743305) OR (dp.fid = 294743306) OR (dp.fid = 294743307) OR (dp.fid = 294743308) OR (dp.fid = 294743309) OR (dp.fid = 294743310) ) ORDER BY RAND() LIMIT 1

118 (133.99124145508): SELECT * FROM jos_deeppockets WHERE 1=1 AND id=294774190 AND published='1' union SELECT * FROM jos_deeppockets_archive WHERE 1=1 AND id=294774190 AND published='1' LIMIT 1

119 (91.075897216797): SELECT * FROM jos_deeppockets WHERE 1=1 AND id=294774190 AND published='1' union SELECT * FROM jos_deeppockets_archive WHERE 1=1 AND id=294774190 AND published='1' LIMIT 1

120 (138.0443572998): SELECT * FROM jos_deeppockets WHERE 1=1 AND id=294774190 AND published='1' union SELECT * FROM jos_deeppockets_archive WHERE 1=1 AND id=294774190 AND published='1' LIMIT 1

121 (66.041946411133): SELECT * FROM jos_deeppockets WHERE 1=1 AND id=294774190 AND published='1' union SELECT * FROM jos_deeppockets_archive WHERE 1=1 AND id=294774190 AND published='1' LIMIT 1

122 (91.075897216797): SELECT * FROM jos_deeppockets WHERE 1=1 AND id=294774190 AND published='1' union SELECT * FROM jos_deeppockets_archive WHERE 1=1 AND id=294774190 AND published='1' LIMIT 1

123 (62.942504882812): SELECT * FROM jos_deeppockets WHERE 1=1 AND id=294774190 AND published='1' union SELECT * FROM jos_deeppockets_archive WHERE 1=1 AND id=294774190 AND published='1' LIMIT 1

124 (336.88545227051): SELECT * FROM trending_articles WHERE `journal_id` = 10 AND `timestamp` <= NOW() + INTERVAL 7 DAY ORDER BY `order`

125 (!!!!! 59993.028640747): SELECT * FROM jos_deeppockets WHERE jos_deeppockets.cat = 10 AND jos_deeppockets.type = 'deeppockets_item' AND jos_deeppockets.published = 1 AND jos_deeppockets.created + INTERVAL 14 DAY >= NOW() ORDER BY jos_deeppockets.hits DESC LIMIT 7

126 (!!!!! 4369.9741363525): SELECT * FROM jos_deeppockets WHERE 1=1 AND id=294774190 AND published='1' union SELECT * FROM jos_deeppockets_archive WHERE 1=1 AND id=294774190 AND published='1' LIMIT 1

127 (111.10305786133): SELECT * FROM jos_deeppockets WHERE 1=1 AND id=294774190 AND published='1' union SELECT * FROM jos_deeppockets_archive WHERE 1=1 AND id=294774190 AND published='1' LIMIT 1

128 (820.87516784668): SELECT fid FROM jos_deeppockets WHERE cat=10 AND id=294774190 AND type='deeppockets_item' AND published='1' union SELECT fid FROM jos_deeppockets_archive WHERE cat=10 AND id=294774190 AND type='deeppockets_item' AND published='1' LIMIT 1

129 (!!!!! 2299482.8224182): ( SELECT dp.id, dp.name, dp.title, dp.video, dp.video_source, dp.created FROM jos_deeppockets AS dp WHERE dp.video != '' AND dp.video_source != '' AND dp.cat = 10 AND dp.published = 1 AND dp.type != 'deeppockets_item_link' ORDER BY created DESC LIMIT 3 ) UNION ( SELECT dpa.id, dpa.name, dpa.title, dpa.video, dpa.video_source, dpa.created FROM jos_deeppockets_archive AS dpa WHERE dpa.video != '' AND dpa.video_source != '' AND dpa.cat = 10 AND dpa.published = 1 AND dpa.type != 'deeppockets_item_link' ORDER BY created DESC LIMIT 3 ) ORDER BY created DESC LIMIT 3

130 (!!!!! 1737853.0502319): ( SELECT dp.id, dp.name, dp.title, dp.video, dp.video_source, dp.created FROM jos_deeppockets AS dp WHERE dp.video != '' AND dp.video_source != '' AND dp.cat = 10 AND dp.published = 1 AND dp.type != 'deeppockets_item_link' ORDER BY created DESC LIMIT 3 ) UNION ( SELECT dpa.id, dpa.name, dpa.title, dpa.video, dpa.video_source, dpa.created FROM jos_deeppockets_archive AS dpa WHERE dpa.video != '' AND dpa.video_source != '' AND dpa.cat = 10 AND dpa.published = 1 AND dpa.type != 'deeppockets_item_link' ORDER BY created DESC LIMIT 3 ) ORDER BY created DESC LIMIT 3

131 (111.81831359863): SELECT dp.id, dp.name, dp.title, dp.video, dp.video_source, dp.created FROM jos_deeppockets AS dp WHERE dp.id = 294800513 LIMIT 1

132 (47.922134399414): SELECT dp.id, dp.name, dp.title, dp.video, dp.video_source, dp.created FROM jos_deeppockets AS dp WHERE dp.id = 294800513 LIMIT 1

133 (46.014785766602): SELECT dp.id, dp.name, dp.title, dp.video, dp.video_source, dp.created FROM jos_deeppockets AS dp WHERE dp.id = 294800359 LIMIT 1

134 (42.200088500977): SELECT dp.id, dp.name, dp.title, dp.video, dp.video_source, dp.created FROM jos_deeppockets AS dp WHERE dp.id = 294800359 LIMIT 1

135 (37.908554077148): SELECT dp.id, dp.name, dp.title, dp.video, dp.video_source, dp.created FROM jos_deeppockets AS dp WHERE dp.id = 294800361 LIMIT 1

136 (36.001205444336): SELECT dp.id, dp.name, dp.title, dp.video, dp.video_source, dp.created FROM jos_deeppockets AS dp WHERE dp.id = 294800361 LIMIT 1

137 (509.97734069824): SELECT * FROM jos_deeppockets WHERE 1=1 AND id=294774190 AND (type='deeppockets_item' OR type='deeppockets_item_link') AND published='1' LIMIT 1

138 (396.0132598877): SELECT * FROM jos_deeppockets_archive WHERE 1=1 AND id=294774190 AND (type='deeppockets_item' OR type='deeppockets_item_link') AND published='1' LIMIT 1

139 (57.93571472168): SELECT name FROM jos_deeppockets WHERE id=215

140 (38.862228393555): SELECT name FROM jos_deeppockets WHERE id=215

141 (624.8950958252): SELECT *, DATE_FORMAT(publish_up,'%d %b %Y') AS date_publish_up, DATE_FORMAT(publish_down,'%d %b %Y') AS date_publish_down FROM jos_events WHERE state=1 AND publish_up > NOW() and extra_info REGEXP '((;)|(^))215((;)|($))' AND catid IN (0,110) ORDER BY publish_up ASC LIMIT 3

142 (580.0724029541): SELECT *, DATE_FORMAT(publish_up,'%d %b %Y') AS date_publish_up, DATE_FORMAT(publish_down,'%d %b %Y') AS date_publish_down FROM jos_events WHERE state=1 AND publish_up > NOW() and extra_info REGEXP '((;)|(^))215((;)|($))' AND catid IN (0,110) ORDER BY publish_up ASC LIMIT 3

143 (!!!!! 1374.9599456787): SELECT * FROM jos_deeppockets WHERE 1=1 AND id=294774190 AND published='1' union SELECT * FROM jos_deeppockets_archive WHERE 1=1 AND id=294774190 AND published='1' LIMIT 1

144 (136.13700866699): SELECT * FROM jos_deeppockets WHERE 1=1 AND id=294774190 AND published='1' union SELECT * FROM jos_deeppockets_archive WHERE 1=1 AND id=294774190 AND published='1' LIMIT 1

145 (!!!!! 9783.0295562744): TRUNCATE TABLE jos_trademed_module

146 (133.99124145508): SELECT dp.*, ch.title as channel, exp.name as author_name, exp.specialization as author_specialization, exp.images as author_photo FROM jos_deeppockets as dp LEFT JOIN jos_experts as exp ON exp.id = dp.author LEFT JOIN jos_deeppockets as ch ON dp.fid = ch.id WHERE 1=1 AND dp.cat=10 AND dp.id=294774190 AND (dp.type='deeppockets_item' OR dp.type='deeppockets_item_link') AND dp.published='1' LIMIT 1

147 (!!!!! 1307.9643249512): SELECT dp.*, ch.title as channel, exp.name as author_name, exp.specialization as author_specialization, exp.images as author_photo FROM jos_deeppockets_archive as dp LEFT JOIN jos_experts as exp ON exp.id = dp.author LEFT JOIN jos_deeppockets as ch ON dp.fid = ch.id WHERE 1=1 AND dp.cat=10 AND dp.id=294774190 AND (dp.type='deeppockets_item' OR dp.type='deeppockets_item_link') AND dp.published='1' LIMIT 1

148 (!!!!! 1111.9842529297): SELECT * FROM jos_deeppockets WHERE 1=1 AND id=294774190 AND (type='deeppockets_item' OR type='deeppockets_item_link') AND published='1' LIMIT 1

149 (116.82510375977): SELECT * FROM jos_deeppockets WHERE 1=1 AND id=294774190 AND (type='deeppockets_item' OR type='deeppockets_item_link') AND published='1' LIMIT 1

150 (87.02278137207): SELECT * FROM jos_deeppockets WHERE 1=1 AND id=294774190 AND published='1' union SELECT * FROM jos_deeppockets_archive WHERE 1=1 AND id=294774190 AND published='1' LIMIT 1

151 (66.041946411133): SELECT * FROM jos_deeppockets WHERE 1=1 AND id=294774190 AND published='1' union SELECT * FROM jos_deeppockets_archive WHERE 1=1 AND id=294774190 AND published='1' LIMIT 1

152 (!!!!! 3035.0685119629): SELECT jos_item.* FROM jos_item INNER JOIN jos_number ON jos_item.numberID=jos_number.id WHERE 1=1 AND (jos_item.imgMini<>'') AND jos_item.type='L' AND jos_item.numberID= ( SELECT jos_number.id FROM jos_number WHERE jos_number.journalID IN (10) and jos_number.id in (select distinct jos_number.id from jos_number join jos_item on jos_item.numberid=jos_number.id where jos_item.type='P') ORDER BY (CONVERT(SUBSTRING(issue,1,2),SIGNED) + (CONVERT(SUBSTRING(issue,3,2),SIGNED)*100)) DESC LIMIT 0,1 ) ORDER BY RAND() DESC LIMIT 6

153 (149.01161193848): SELECT * FROM jos_deeppockets WHERE 1=1 AND id=294774190 AND published='1' union SELECT * FROM jos_deeppockets_archive WHERE 1=1 AND id=294774190 AND published='1' LIMIT 1

154 (71.048736572266): SELECT * FROM jos_deeppockets WHERE 1=1 AND id=294774190 AND published='1' union SELECT * FROM jos_deeppockets_archive WHERE 1=1 AND id=294774190 AND published='1' LIMIT 1

155 (!!!!! 3678.0834197998): SELECT fid FROM jos_deeppockets WHERE cat=10 AND id=294774190 AND type='deeppockets_item' AND published='1' union SELECT fid FROM jos_deeppockets_archive WHERE cat=10 AND id=294774190 AND type='deeppockets_item' AND published='1' LIMIT 1

156 (163.07830810547): select distinct links.* from jos_textlinks links join jos_textlinks_placement placement on links.id=placement.link_id where journal_id=(10) and (channel_id=215 or channel_id is null)

157 (126.12342834473): select distinct links.* from jos_textlinks links join jos_textlinks_placement placement on links.id=placement.link_id where journal_id=(10) and (channel_id=215 or channel_id is null)

158 (89.883804321289): SELECT livesite FROM jos_dp_categories WHERE id=10 AND published='1' LIMIT 1

159 (211.00044250488): SELECT * FROM jos_deeppockets WHERE 1=1 AND id=294774190 AND published='1' union SELECT * FROM jos_deeppockets_archive WHERE 1=1 AND id=294774190 AND published='1' LIMIT 1

160 (129.93812561035): SELECT * FROM jos_deeppockets WHERE 1=1 AND id=294774190 AND published='1' union SELECT * FROM jos_deeppockets_archive WHERE 1=1 AND id=294774190 AND published='1' LIMIT 1

161 (133.99124145508): select jos_twitters.link from jos_twitters where cat=10

162 (90.837478637695): select jos_facebook.link from jos_facebook where cat=10

163 (76.055526733398): select jos_google_plus.link from jos_google_plus where cat=10

164 (694.03648376465): select jos_linkedin.link from jos_linkedin where cat=10

165 (453.94897460938): update jos_bannersmanager_banner set impmade=impmade+1 where id in(14615,14202,14203)

166 (!!!!! 5861.0439300537): insert into jos_banners_delta values (14615,'2025-04-30 12:32:06','3.14.132.123','view'),(14202,'2025-04-30 12:32:06','3.14.132.123','view'),(14203,'2025-04-30 12:32:06','3.14.132.123','view')

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