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Early Changes Leading to Breast Tumors Revealed by Spectroscopic Imaging

By MedImaging International staff writers
Posted on 26 Mar 2012
Image: A live human mammary gland structure created in research that uses a new imaging technology to reveal subtle changes in breast tissue, representing a potential tool to determine a woman's risk of developing breast cancer and to study ways of preventing the disease. Unlike conventional cell cultures, the 3-D cultures have the round shape of milk-producing glands and behave like real tissue (Photo courtesy of Purdue University /Shuhua Yue).
Image: A live human mammary gland structure created in research that uses a new imaging technology to reveal subtle changes in breast tissue, representing a potential tool to determine a woman's risk of developing breast cancer and to study ways of preventing the disease. Unlike conventional cell cultures, the 3-D cultures have the round shape of milk-producing glands and behave like real tissue (Photo courtesy of Purdue University /Shuhua Yue).
Researchers have created a new imaging technology that reveals slight changes in breast tissue, representing a potential tool to determine a woman’s risk of developing breast cancer and to assess ways of preventing the disease.

The researchers, using a special three-dimensional (3D) culture that mimics living mammary gland tissue, also showed that a fatty acid found in some foods influences this early precancerous stage. Unlike traditional cell cultures, which are flat, the 3D cultures have the round shape of milk-producing glands and behave like real tissue, according to Dr. Sophie Lelièvre an associate professor of basic medical sciences at Purdue University (West Lafayette, IN, USA).

Researchers are studying changes that take place in epithelial cells, which make up tissues and organs where 90% of cancers occur. The changes in breast tissue are thought to be necessary for tumors to form, she said. “By mimicking the early stage conducive to tumors and using a new imaging tool, our goal is to be able to measure this change and then take steps to prevent it,” Dr. Lelièvre said.

The new imaging technique, called vibrational spectral microscopy, can be used to identify and track certain molecules by measuring their vibration with a laser. Whereas other imaging tools may take days to get results, the new method works at high speed, enabling researchers to measure changes in real time in live tissue, according to Dr. Ji-Xin Cheng, an associate professor of biomedical engineering and chemistry. By monitoring the same three-dimensional (3D) culture before and after exposure to certain risk factors, the new method enables researchers to detect subtle changes in single live cells, he reported.

Findings are detailed in a research paper that appeared March 7, 2012, in the Biophysical Journal. The article was written by doctoral students Shuhua Yue, Juan Manuel Cárdenas-Mora, and Lesley S. Chaboub, Lelièvre and Dr. Cheng. “This work shows the importance of engineering for the development of primary prevention research in breast cancer,” said Mr. Yue, a biomedical engineering student whose work was funded through a fellowship from the US Department of Defense Breast Cancer Research Program.

Researchers evaluated live tissue in a culture that reproduces the mammary epithelium. “This extremely sensitive technique shows the harmful impact of a nutrient called arachidonic acid,” said Dr. Lelièvre, associate director of discovery groups at the Purdue Center for Cancer Research. “This fatty acid has been previously proposed to increase breast cancer risk, but until now there was no biological evidence of what it could do to alter breast epithelial cells."

The imaging method identifies changes in the “basoapical polarity” of epithelial tissues. Specific proteins and other biochemical compounds called lipids are typically located in one of two regions, called the basal and apical membranes. Because only specific proteins and lipids are found in basal membranes, while others are located only in apical membranes, the cells are reported to be polarized.

“This polarity is critical for the proper structure and function of tissue,” Dr. Lelièvre said. “What we have shown previously is that when polarity is altered, tissue that otherwise looks normal can be pushed into a cell cycle necessary to form a tumor. It’s the earliest change in the epithelial tissue that puts the cells at risk to form a tumor. Now, thanks to the vibrational spectral microscopy technique developed by Dr. Cheng, we can measure apical polarity status in live tissues and real time.”

The findings could lead to a way to prevent tumor formation by restoring the correct polarity. “We are mimicking formation of breast epithelium as it is normally polarized, and we can play with it and make it lose apical polarity on demand,” Dr. Lelièvre said. “Then we can mimic an early change thought to be conducive to tumor development.”

The researchers’ goal is to use the imaging technology on live 3D cultures of noncancerous breast tissue to screen for protective and risk factors for breast cancer the same way that tumors are used now in cultures to screen for drugs that can be used for treatment. “Now there is no good way to assess risk for breast cancer,” Dr. Lelièvre said. “Assessments are mainly based on family history and genetic changes, and this only accounts for a very small percentage of women who get breast cancer. We need technologies to assess the risk better and then screen for protective factors that could be used on individual patients because not everybody will be responsive to the same factors.”

An immediate application of the technique is a way to study cell lines created from tissue taken from women at different risk levels, screening for factors that could restore full polarity in the breast tissue to prevent tumor formation. “The state of the art is to take tumor cells, put them in a 3D culture where they form tumors, and then test drugs on these tumors,” Dr. Lelièvre said. “What we want to do now is the same thing but for preventive strategies using a normal tissue.”

In a collaboration with researchers at Indiana University-Purdue University Indianapolis, the team plans to study cell lines from women who are at different risk levels. The new imaging tool was needed because traditional live cell imaging methods are designed for flat cell cultures. Mimicking tissue polarity requires a 3D culture to form the mammary gland structures at the end of the breast ducts. The structures resemble tiny balls about 30 micrometers in diameter and contain approximately 35 cells.

Dr. Cheng reported that his lab will continue to develop a “hyperspectral” imaging system capable of not only imaging a specific area in a culture but also many locations to form a point-by-point map so that tissue polarity can be directly visualized.

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72 (45.061111450195): SELECT id,name,title FROM jos_deeppockets WHERE cat=10 AND TYPE='file_folder' AND published=1 AND id != 294743300 AND id != 294743301 AND id != 294743302 AND id != 294743303 AND id != 294743304 AND id != 294743305 AND id != 294743306 AND id != 294743307 AND id != 294743308 AND id != 294743309 AND id != 294743310 AND metakey != 'aacc' ORDER BY `ordering`
73 (52.928924560547): SELECT show_live FROM jos_com_show_setting LIMIT 1
74 (43.153762817383): SELECT show_live FROM jos_com_show_setting LIMIT 1
75 (42.915344238281): SELECT show_live FROM jos_com_show_setting LIMIT 1
76 (31.948089599609): SELECT show_live FROM jos_com_show_setting LIMIT 1
77 (31.232833862305): SELECT show_live FROM jos_com_show_setting LIMIT 1
78 (30.994415283203): SELECT show_live FROM jos_com_show_setting LIMIT 1
79 (30.040740966797): SELECT show_live FROM jos_com_show_setting LIMIT 1
80 (30.994415283203): SELECT show_live FROM jos_com_show_setting LIMIT 1
81 (30.040740966797): SELECT show_live FROM jos_com_show_setting LIMIT 1
82 (30.994415283203): SELECT show_live FROM jos_com_show_setting LIMIT 1
83 (29.802322387695): SELECT show_live FROM jos_com_show_setting LIMIT 1
84 (30.994415283203): SELECT show_live FROM jos_com_show_setting LIMIT 1
85 (28.848648071289): SELECT show_live FROM jos_com_show_setting LIMIT 1
86 (30.040740966797): SELECT show_live FROM jos_com_show_setting LIMIT 1
87 (46.968460083008): SELECT fid FROM jos_deeppockets WHERE cat=10 AND id=294739928 AND (type='deeppockets_item' OR type='deeppockets_item_link') AND published='1' LIMIT 1
88 (37.908554077148): SELECT fid FROM jos_deeppockets_archive WHERE cat=10 AND id=294739928 AND (type='deeppockets_item' OR type='deeppockets_item_link') AND published='1' LIMIT 1
89 (43.153762817383): SELECT id,name,title FROM jos_deeppockets WHERE cat=10 AND TYPE='file_folder' AND published=1 AND id != 294743300 AND id != 294743301 AND id != 294743302 AND id != 294743303 AND id != 294743304 AND id != 294743305 AND id != 294743306 AND id != 294743307 AND id != 294743308 AND id != 294743309 AND id != 294743310 AND metakey != 'aacc' ORDER BY `ordering`
90 (48.87580871582): SELECT show_live FROM jos_com_show_setting LIMIT 1
91 (28.848648071289): SELECT show_live FROM jos_com_show_setting LIMIT 1
92 (28.133392333984): SELECT show_live FROM jos_com_show_setting LIMIT 1
93 (28.133392333984): SELECT show_live FROM jos_com_show_setting LIMIT 1
94 (26.941299438477): SELECT show_live FROM jos_com_show_setting LIMIT 1
95 (27.894973754883): SELECT show_live FROM jos_com_show_setting LIMIT 1
96 (34.809112548828): SELECT show_live FROM jos_com_show_setting LIMIT 1
97 (28.133392333984): SELECT show_live FROM jos_com_show_setting LIMIT 1
98 (26.941299438477): SELECT show_live FROM jos_com_show_setting LIMIT 1
99 (27.894973754883): SELECT show_live FROM jos_com_show_setting LIMIT 1
100 (28.133392333984): SELECT show_live FROM jos_com_show_setting LIMIT 1
101 (29.087066650391): SELECT show_live FROM jos_com_show_setting LIMIT 1
102 (28.133392333984): SELECT show_live FROM jos_com_show_setting LIMIT 1
103 (27.179718017578): SELECT show_live FROM jos_com_show_setting LIMIT 1
104 (99.897384643555): SELECT * FROM jos_deeppockets WHERE 1=1 AND id=294739928 AND published='1' union SELECT * FROM jos_deeppockets_archive WHERE 1=1 AND id=294739928 AND published='1' LIMIT 1
105 (65.088272094727): SELECT * FROM jos_deeppockets WHERE 1=1 AND id=294739928 AND published='1' union SELECT * FROM jos_deeppockets_archive WHERE 1=1 AND id=294739928 AND published='1' LIMIT 1
106 (49.114227294922): SELECT dp.*, ch.title as channel, exp.name as author_name, exp.specialization as author_specialization, exp.images as author_photo FROM jos_deeppockets as dp LEFT JOIN jos_experts as exp ON exp.id = dp.author LEFT JOIN jos_deeppockets as ch ON dp.fid = ch.id WHERE 1=1 AND dp.cat=10 AND dp.id=294739928 AND (dp.type='deeppockets_item' OR dp.type='deeppockets_item_link') AND dp.published='1' LIMIT 1
107 (50.067901611328): SELECT dp.*, ch.title as channel, exp.name as author_name, exp.specialization as author_specialization, exp.images as author_photo FROM jos_deeppockets_archive as dp LEFT JOIN jos_experts as exp ON exp.id = dp.author LEFT JOIN jos_deeppockets as ch ON dp.fid = ch.id WHERE 1=1 AND dp.cat=10 AND dp.id=294739928 AND (dp.type='deeppockets_item' OR dp.type='deeppockets_item_link') AND dp.published='1' LIMIT 1
108 (82.015991210938): SELECT * FROM jos_deeppockets WHERE 1=1 AND id=294739928 AND published='1' union SELECT * FROM jos_deeppockets_archive WHERE 1=1 AND id=294739928 AND published='1' LIMIT 1
109 (55.074691772461): SELECT * FROM jos_deeppockets WHERE 1=1 AND id=294739928 AND published='1' union SELECT * FROM jos_deeppockets_archive WHERE 1=1 AND id=294739928 AND published='1' LIMIT 1
110 (47.922134399414): SELECT dp.*, ch.title as channel, exp.name as author_name, exp.specialization as author_specialization, exp.images as author_photo FROM jos_deeppockets as dp LEFT JOIN jos_experts as exp ON exp.id = dp.author LEFT JOIN jos_deeppockets as ch ON dp.fid = ch.id WHERE 1=1 AND dp.id=294739928 AND (dp.type='deeppockets_item' OR dp.type='deeppockets_item_link') AND dp.published='1' LIMIT 1
111 (364.06517028809): SELECT dp.*, ch.title as channel, exp.name as author_name, exp.specialization as author_specialization, exp.images as author_photo FROM jos_deeppockets_archive as dp LEFT JOIN jos_experts as exp ON exp.id = dp.author LEFT JOIN jos_deeppockets as ch ON dp.fid = ch.id WHERE 1=1 AND dp.id=294739928 AND (dp.type='deeppockets_item' OR dp.type='deeppockets_item_link') AND dp.published='1' LIMIT 1
112 (538.82598876953): SELECT dp.name, dp.id, dp2.name as channel, ex.name as author_name, ex.specialization, ex.images FROM jos_deeppockets as dp LEFT JOIN jos_experts as ex ON ex.id = dp.author LEFT JOIN jos_deeppockets as dp2 ON dp2.id = dp.fid WHERE dp.author != 0 AND dp.cat = 10 AND dp.published = 1 AND ( (dp.fid = 294743300) OR (dp.fid = 294743301) OR (dp.fid = 294743302) OR (dp.fid = 294743303) OR (dp.fid = 294743304) OR (dp.fid = 294743305) OR (dp.fid = 294743306) OR (dp.fid = 294743307) OR (dp.fid = 294743308) OR (dp.fid = 294743309) OR (dp.fid = 294743310) ) ORDER BY RAND() LIMIT 1
113 (410.79521179199): SELECT dp.name, dp.id, dp2.name as channel, ex.name as author_name, ex.specialization, ex.images FROM jos_deeppockets as dp LEFT JOIN jos_experts as ex ON ex.id = dp.author LEFT JOIN jos_deeppockets as dp2 ON dp2.id = dp.fid WHERE dp.author != 0 AND dp.cat = 10 AND dp.published = 1 AND ( (dp.fid = 294743300) OR (dp.fid = 294743301) OR (dp.fid = 294743302) OR (dp.fid = 294743303) OR (dp.fid = 294743304) OR (dp.fid = 294743305) OR (dp.fid = 294743306) OR (dp.fid = 294743307) OR (dp.fid = 294743308) OR (dp.fid = 294743309) OR (dp.fid = 294743310) ) ORDER BY RAND() LIMIT 1
114 (113.01040649414): SELECT * FROM jos_deeppockets WHERE 1=1 AND id=294739928 AND published='1' union SELECT * FROM jos_deeppockets_archive WHERE 1=1 AND id=294739928 AND published='1' LIMIT 1
115 (73.909759521484): SELECT * FROM jos_deeppockets WHERE 1=1 AND id=294739928 AND published='1' union SELECT * FROM jos_deeppockets_archive WHERE 1=1 AND id=294739928 AND published='1' LIMIT 1
116 (87.02278137207): SELECT * FROM jos_deeppockets WHERE 1=1 AND id=294739928 AND published='1' union SELECT * FROM jos_deeppockets_archive WHERE 1=1 AND id=294739928 AND published='1' LIMIT 1
117 (54.836273193359): SELECT * FROM jos_deeppockets WHERE 1=1 AND id=294739928 AND published='1' union SELECT * FROM jos_deeppockets_archive WHERE 1=1 AND id=294739928 AND published='1' LIMIT 1
118 (68.902969360352): SELECT * FROM jos_deeppockets WHERE 1=1 AND id=294739928 AND published='1' union SELECT * FROM jos_deeppockets_archive WHERE 1=1 AND id=294739928 AND published='1' LIMIT 1
119 (54.121017456055): SELECT * FROM jos_deeppockets WHERE 1=1 AND id=294739928 AND published='1' union SELECT * FROM jos_deeppockets_archive WHERE 1=1 AND id=294739928 AND published='1' LIMIT 1
120 (79.154968261719): SELECT * FROM jos_deeppockets WHERE 1=1 AND id=294739928 AND published='1' union SELECT * FROM jos_deeppockets_archive WHERE 1=1 AND id=294739928 AND published='1' LIMIT 1
121 (41.961669921875): SELECT * FROM jos_deeppockets WHERE 1=1 AND id=294739928 AND published='1' union SELECT * FROM jos_deeppockets_archive WHERE 1=1 AND id=294739928 AND published='1' LIMIT 1
122 (258.9225769043): SELECT * FROM trending_articles WHERE `journal_id` = 10 AND `timestamp` <= NOW() + INTERVAL 7 DAY ORDER BY `order`
123 (!!!!! 20435.094833374): SELECT * FROM jos_deeppockets WHERE jos_deeppockets.cat = 10 AND jos_deeppockets.type = 'deeppockets_item' AND jos_deeppockets.published = 1 AND jos_deeppockets.created + INTERVAL 14 DAY >= NOW() ORDER BY jos_deeppockets.hits DESC LIMIT 7
124 (529.05082702637): SELECT fid FROM jos_deeppockets WHERE cat=10 AND id=294739928 AND type='deeppockets_item' AND published='1' union SELECT fid FROM jos_deeppockets_archive WHERE cat=10 AND id=294739928 AND type='deeppockets_item' AND published='1' LIMIT 1
125 (!!!!! 7406.9499969482): ( SELECT dp.id, dp.name, dp.title, dp.video, dp.video_source, dp.created FROM jos_deeppockets AS dp WHERE dp.video != '' AND dp.video_source != '' AND dp.cat = 10 AND dp.published = 1 AND dp.type != 'deeppockets_item_link' ORDER BY created DESC LIMIT 3 ) UNION ( SELECT dpa.id, dpa.name, dpa.title, dpa.video, dpa.video_source, dpa.created FROM jos_deeppockets_archive AS dpa WHERE dpa.video != '' AND dpa.video_source != '' AND dpa.cat = 10 AND dpa.published = 1 AND dpa.type != 'deeppockets_item_link' ORDER BY created DESC LIMIT 3 ) ORDER BY created DESC LIMIT 3
126 (71.048736572266): ( SELECT dp.id, dp.name, dp.title, dp.video, dp.video_source, dp.created FROM jos_deeppockets AS dp WHERE dp.video != '' AND dp.video_source != '' AND dp.cat = 10 AND dp.published = 1 AND dp.type != 'deeppockets_item_link' ORDER BY created DESC LIMIT 3 ) UNION ( SELECT dpa.id, dpa.name, dpa.title, dpa.video, dpa.video_source, dpa.created FROM jos_deeppockets_archive AS dpa WHERE dpa.video != '' AND dpa.video_source != '' AND dpa.cat = 10 AND dpa.published = 1 AND dpa.type != 'deeppockets_item_link' ORDER BY created DESC LIMIT 3 ) ORDER BY created DESC LIMIT 3
127 (183.10546875): SELECT dp.id, dp.name, dp.title, dp.video, dp.video_source, dp.created FROM jos_deeppockets AS dp WHERE dp.id = 294803337 LIMIT 1
128 (34.093856811523): SELECT dp.id, dp.name, dp.title, dp.video, dp.video_source, dp.created FROM jos_deeppockets AS dp WHERE dp.id = 294803337 LIMIT 1
129 (159.97886657715): SELECT dp.id, dp.name, dp.title, dp.video, dp.video_source, dp.created FROM jos_deeppockets AS dp WHERE dp.id = 294803276 LIMIT 1
130 (78.916549682617): SELECT dp.id, dp.name, dp.title, dp.video, dp.video_source, dp.created FROM jos_deeppockets AS dp WHERE dp.id = 294803276 LIMIT 1
131 (117.06352233887): SELECT dp.id, dp.name, dp.title, dp.video, dp.video_source, dp.created FROM jos_deeppockets_archive AS dp WHERE dp.id = 294803276 LIMIT 1
132 (32.901763916016): SELECT dp.id, dp.name, dp.title, dp.video, dp.video_source, dp.created FROM jos_deeppockets_archive AS dp WHERE dp.id = 294803276 LIMIT 1
133 (107.0499420166): SELECT dp.id, dp.name, dp.title, dp.video, dp.video_source, dp.created FROM jos_deeppockets AS dp WHERE dp.id = 294802958 LIMIT 1
134 (30.994415283203): SELECT dp.id, dp.name, dp.title, dp.video, dp.video_source, dp.created FROM jos_deeppockets AS dp WHERE dp.id = 294802958 LIMIT 1
135 (410.07995605469): SELECT * FROM jos_deeppockets WHERE 1=1 AND id=294739928 AND (type='deeppockets_item' OR type='deeppockets_item_link') AND published='1' LIMIT 1
136 (288.96331787109): SELECT * FROM jos_deeppockets_archive WHERE 1=1 AND id=294739928 AND (type='deeppockets_item' OR type='deeppockets_item_link') AND published='1' LIMIT 1
137 (169.03877258301): SELECT name FROM jos_deeppockets WHERE id=215
138 (31.948089599609): SELECT name FROM jos_deeppockets WHERE id=215
139 (613.92784118652): SELECT *, DATE_FORMAT(publish_up,'%d %b %Y') AS date_publish_up, DATE_FORMAT(publish_down,'%d %b %Y') AS date_publish_down FROM jos_events WHERE state=1 AND publish_up > NOW() and extra_info REGEXP '((;)|(^))215((;)|($))' AND catid IN (0,110) ORDER BY publish_up ASC LIMIT 3
140 (454.90264892578): SELECT *, DATE_FORMAT(publish_up,'%d %b %Y') AS date_publish_up, DATE_FORMAT(publish_down,'%d %b %Y') AS date_publish_down FROM jos_events WHERE state=1 AND publish_up > NOW() and extra_info REGEXP '((;)|(^))215((;)|($))' AND catid IN (0,110) ORDER BY publish_up ASC LIMIT 3
141 (72.956085205078): SELECT * FROM jos_deeppockets WHERE 1=1 AND id=294739928 AND published='1' union SELECT * FROM jos_deeppockets_archive WHERE 1=1 AND id=294739928 AND published='1' LIMIT 1
142 (51.021575927734): SELECT * FROM jos_deeppockets WHERE 1=1 AND id=294739928 AND published='1' union SELECT * FROM jos_deeppockets_archive WHERE 1=1 AND id=294739928 AND published='1' LIMIT 1
143 (!!!!! 8610.0101470947): TRUNCATE TABLE jos_trademed_module
144 (108.00361633301): SELECT dp.*, ch.title as channel, exp.name as author_name, exp.specialization as author_specialization, exp.images as author_photo FROM jos_deeppockets as dp LEFT JOIN jos_experts as exp ON exp.id = dp.author LEFT JOIN jos_deeppockets as ch ON dp.fid = ch.id WHERE 1=1 AND dp.cat=10 AND dp.id=294739928 AND (dp.type='deeppockets_item' OR dp.type='deeppockets_item_link') AND dp.published='1' LIMIT 1
145 (75.101852416992): SELECT dp.*, ch.title as channel, exp.name as author_name, exp.specialization as author_specialization, exp.images as author_photo FROM jos_deeppockets_archive as dp LEFT JOIN jos_experts as exp ON exp.id = dp.author LEFT JOIN jos_deeppockets as ch ON dp.fid = ch.id WHERE 1=1 AND dp.cat=10 AND dp.id=294739928 AND (dp.type='deeppockets_item' OR dp.type='deeppockets_item_link') AND dp.published='1' LIMIT 1
146 (998.02017211914): SELECT * FROM jos_deeppockets WHERE 1=1 AND id=294739928 AND (type='deeppockets_item' OR type='deeppockets_item_link') AND published='1' LIMIT 1
147 (56.028366088867): SELECT * FROM jos_deeppockets WHERE 1=1 AND id=294739928 AND (type='deeppockets_item' OR type='deeppockets_item_link') AND published='1' LIMIT 1
148 (!!!!! 1188.0397796631): SELECT * FROM jos_deeppockets WHERE 1=1 AND id=294739928 AND published='1' union SELECT * FROM jos_deeppockets_archive WHERE 1=1 AND id=294739928 AND published='1' LIMIT 1
149 (57.93571472168): SELECT * FROM jos_deeppockets WHERE 1=1 AND id=294739928 AND published='1' union SELECT * FROM jos_deeppockets_archive WHERE 1=1 AND id=294739928 AND published='1' LIMIT 1
150 (!!!!! 1044.9886322021): SELECT jos_item.* FROM jos_item INNER JOIN jos_number ON jos_item.numberID=jos_number.id WHERE 1=1 AND (jos_item.imgMini<>'') AND jos_item.type='L' AND jos_item.numberID= ( SELECT jos_number.id FROM jos_number WHERE jos_number.journalID IN (10) and jos_number.id in (select distinct jos_number.id from jos_number join jos_item on jos_item.numberid=jos_number.id where jos_item.type='P') ORDER BY (CONVERT(SUBSTRING(issue,1,2),SIGNED) + (CONVERT(SUBSTRING(issue,3,2),SIGNED)*100)) DESC LIMIT 0,1 ) ORDER BY RAND() DESC LIMIT 6
151 (493.04962158203): SELECT * FROM jos_deeppockets WHERE 1=1 AND id=294739928 AND published='1' union SELECT * FROM jos_deeppockets_archive WHERE 1=1 AND id=294739928 AND published='1' LIMIT 1
152 (139.95170593262): SELECT * FROM jos_deeppockets WHERE 1=1 AND id=294739928 AND published='1' union SELECT * FROM jos_deeppockets_archive WHERE 1=1 AND id=294739928 AND published='1' LIMIT 1
153 (41.961669921875): SELECT fid FROM jos_deeppockets WHERE cat=10 AND id=294739928 AND type='deeppockets_item' AND published='1' union SELECT fid FROM jos_deeppockets_archive WHERE cat=10 AND id=294739928 AND type='deeppockets_item' AND published='1' LIMIT 1
154 (79.154968261719): select distinct links.* from jos_textlinks links join jos_textlinks_placement placement on links.id=placement.link_id where journal_id=(10) and (channel_id=215 or channel_id is null)
155 (31.948089599609): select distinct links.* from jos_textlinks links join jos_textlinks_placement placement on links.id=placement.link_id where journal_id=(10) and (channel_id=215 or channel_id is null)
156 (40.054321289062): SELECT livesite FROM jos_dp_categories WHERE id=10 AND published='1' LIMIT 1
157 (243.18695068359): SELECT * FROM jos_deeppockets WHERE 1=1 AND id=294739928 AND published='1' union SELECT * FROM jos_deeppockets_archive WHERE 1=1 AND id=294739928 AND published='1' LIMIT 1
158 (64.13459777832): SELECT * FROM jos_deeppockets WHERE 1=1 AND id=294739928 AND published='1' union SELECT * FROM jos_deeppockets_archive WHERE 1=1 AND id=294739928 AND published='1' LIMIT 1
159 (61.03515625): select jos_twitters.link from jos_twitters where cat=10
160 (30.040740966797): select jos_facebook.link from jos_facebook where cat=10
161 (30.040740966797): select jos_google_plus.link from jos_google_plus where cat=10
162 (29.087066650391): select jos_linkedin.link from jos_linkedin where cat=10
163 (326.87187194824): update jos_bannersmanager_banner set impmade=impmade+1 where id in(14202,14203)
164 (!!!!! 1113.8916015625): insert into jos_banners_delta values (14202,'2025-06-24 02:21:08','216.73.216.120','view'),(14203,'2025-06-24 02:21:08','216.73.216.120','view')