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Stitching Software Creates Virtual 3D Tissue Maps

By MedImaging International staff writers
Posted on 21 Aug 2019
Image: A virtual cross-section of the complete nervous system of a fruit fly larva (Photo courtesy of Janelia/ MDC).
Image: A virtual cross-section of the complete nervous system of a fruit fly larva (Photo courtesy of Janelia/ MDC).
A new study shows how pioneering software reconstructs tissue slides into a navigable, three-dimensional (3D) organ model that can illustrate cellular functions such as gene expression.

Developed by researchers at Ludwig-Maximilians-Universität (LMU; Munich, Germany), Max Delbrück Center for Molecular Medicine (MDC; Berlin, Germany), Howard Hughes Medical Institute (HHMI; Ashburn, USA), and other institutions, BigStitcher allows simple and efficient alignment of image datasets acquired by lightsheet, widefield, or confocal microscopes. Images of almost arbitrary size are supported, ranging from very small images up to volumes in the range of many terabytes.

Dubbed by the researchers as “Google Maps in 3D”, BigStitcher enables interactive visualization, fast and precise alignment, spatially resolved quality estimation, real-time fusion, and deconvolution of dual-illumination, multi-tile, multiview datasets at any level of detail desired. The converted images can also be rotated and turned virtually, providing an overview of the entire sample, or zoomed into the level of individual structures, and even systematically characterized at the single-cell level. The software also compensates for optical effects, improving accuracy and enabling subsequent biological analysis.

Other features include integrated downstream processing of the image data; pre-selection of the best illumination direction at every image block in the sample; support of non-regular acquisition grids, which includes 'intelligent acquisitions' where some of the image blocks can be missing if only background is present; and support of image data acquired at different resolutions to combine overview scans with high resolution acquisitions of specific areas of interest. BigStitcher is being distributed within the Fiji framework, and can be downloaded and used free of charge. The study was published on August 5, 2019, in Nature Methods.

“One can not only get an overview of the big picture, but can also zoom in to specifically examine individual structures at the desired resolution,” said senior author Stephan Preibisch, PhD, head of the MDC research group on Microscopy, Image Analysis & Modeling of Developing Organisms. “The software automatically assesses the quality of the acquired data. The brighter a particular region of, say, a mouse brain or a human organ is displayed on screen, the higher the validity and reliability of the acquired data.”

Related Links:
Ludwig-Maximilians-Universität
Max Delbrück Center for Molecular Medicine
Howard Hughes Medical Institute

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		LIMIT 1
49
(66.995620727539): SELECT * FROM jos_deeppockets WHERE 1=1 AND id=294779078 AND published='1'
				union
		SELECT * FROM jos_deeppockets_archive WHERE 1=1 AND id=294779078 AND published='1'
		LIMIT 1
50
(94.890594482422): SELECT * FROM jos_deeppockets WHERE 1=1 AND id=294779078 AND published='1'
				union
		SELECT * FROM jos_deeppockets_archive WHERE 1=1 AND id=294779078 AND published='1'
		LIMIT 1
51
(56.028366088867): SELECT * FROM jos_deeppockets WHERE 1=1 AND id=294779078 AND published='1'
				union
		SELECT * FROM jos_deeppockets_archive WHERE 1=1 AND id=294779078 AND published='1'
		LIMIT 1
52
(346.89903259277): SELECT name FROM jos_deeppockets WHERE id=294779078 LIMIT 1
53
(277.99606323242): SELECT name FROM jos_deeppockets_archive WHERE id=294779078 LIMIT 1
54
(70.810317993164): SELECT a.*
 FROM jos_components AS a
 WHERE ( a.admin_menu_link = 'option=com_syndicate' OR a.admin_menu_link = 'option=com_syndicate&hidemainmenu=1' )
 AND a.option = 'com_syndicate'
55
(89.883804321289): SELECT dp.*, ch.title as channel, exp.name as author_name, exp.specialization as author_specialization, exp.images as author_photo FROM jos_deeppockets as dp
							LEFT JOIN jos_experts as exp
							ON exp.id = dp.author
							LEFT JOIN jos_deeppockets as ch
							ON dp.fid = ch.id
							WHERE 1=1
							 AND dp.cat=10 
							AND dp.id=294779078
							AND (dp.type='deeppockets_item' OR dp.type='deeppockets_item_link')
							AND dp.published='1' LIMIT 1
56
(557.18421936035): SELECT dp.*, ch.title as channel, exp.name as author_name, exp.specialization as author_specialization, exp.images as author_photo FROM jos_deeppockets_archive as dp
								LEFT JOIN jos_experts as exp
								ON exp.id = dp.author
								LEFT JOIN jos_deeppockets as ch
								ON dp.fid = ch.id
								WHERE 1=1
								 AND dp.cat=10 
								AND dp.id=294779078
								AND (dp.type='deeppockets_item' OR dp.type='deeppockets_item_link')
								AND dp.published='1' LIMIT 1
57
(!!!!! 1281.0230255127): SELECT * FROM jos_deeppockets WHERE 1=1 AND id=294779078 AND published='1'
				union
		SELECT * FROM jos_deeppockets_archive WHERE 1=1 AND id=294779078 AND published='1'
		LIMIT 1
58
(125.16975402832): SELECT * FROM jos_deeppockets WHERE 1=1 AND id=294779078 AND published='1'
				union
		SELECT * FROM jos_deeppockets_archive WHERE 1=1 AND id=294779078 AND published='1'
		LIMIT 1
59
(926.97143554688): SELECT * FROM jos_deeppockets WHERE 1=1 AND id=294779078 AND published='1'
				union
		SELECT * FROM jos_deeppockets_archive WHERE 1=1 AND id=294779078 AND published='1'
		LIMIT 1
60
(101.08947753906): SELECT * FROM jos_deeppockets WHERE 1=1 AND id=294779078 AND published='1'
				union
		SELECT * FROM jos_deeppockets_archive WHERE 1=1 AND id=294779078 AND published='1'
		LIMIT 1
61
(96.08268737793): select cover from jos_number
where journalid in (5) and cover is not null
order by left(issue,2)+right(issue,2)*12 desc limit 1
62
(389.09912109375): SELECT fid FROM jos_deeppockets WHERE id=294779078 AND published='1' union SELECT fid FROM jos_deeppockets_archive WHERE id=294779078 AND published='1'  LIMIT 1
63
(58.889389038086): SELECT id,name,sponsor_text
                    FROM jos_deeppockets dp
					left outer join jos_channel_sponsor cs
					on dp.id=cs.fid
                    WHERE cat=10
                    AND TYPE='file_folder'
                    AND published=1
                    AND id=215
                    ORDER BY `ordering`
64
(36.954879760742): select jos_twitters.link from jos_twitters where cat=10
65
(26.941299438477): select jos_facebook.link from jos_facebook where cat=10
66
(26.941299438477): select jos_google_plus.link from jos_google_plus where cat=10
67
(28.133392333984): select jos_linkedin.link from jos_linkedin where cat=10
68
(27.894973754883): SELECT fid FROM jos_deeppockets WHERE id=294779078 AND published='1' union SELECT fid FROM jos_deeppockets_archive WHERE id=294779078 AND published='1'  LIMIT 1
69
(28.848648071289): SELECT id,name,sponsor_text
                    FROM jos_deeppockets dp
					left outer join jos_channel_sponsor cs
					on dp.id=cs.fid
                    WHERE cat=10
                    AND TYPE='file_folder'
                    AND published=1
                    AND id=215
                    ORDER BY `ordering`
70
(246.04797363281): SELECT fid FROM jos_deeppockets
								WHERE cat=10
								AND id=294779078
								AND (type='deeppockets_item' OR type='deeppockets_item_link') 
								AND published='1' 
								LIMIT 1
71
(147.10426330566): SELECT fid FROM jos_deeppockets_archive WHERE cat=10 AND id=294779078 AND (type='deeppockets_item' OR type='deeppockets_item_link') AND published='1' LIMIT 1
72
(41.007995605469): SELECT id,name,title
 FROM jos_deeppockets WHERE cat=10 AND TYPE='file_folder' AND published=1
			AND id != 294743300
			AND id != 294743301
			AND id != 294743302
			AND id != 294743303
			AND id != 294743304
			AND id != 294743305
			AND id != 294743306
			AND id != 294743307
			AND id != 294743308
			AND id != 294743309
			AND id != 294743310
			AND metakey != 'aacc'
		ORDER BY `ordering`
73
(61.988830566406): SELECT show_live FROM jos_com_show_setting LIMIT 1
74
(30.994415283203): SELECT show_live FROM jos_com_show_setting LIMIT 1
75
(36.954879760742): SELECT show_live FROM jos_com_show_setting LIMIT 1
76
(37.908554077148): SELECT show_live FROM jos_com_show_setting LIMIT 1
77
(79.870223999023): SELECT show_live FROM jos_com_show_setting LIMIT 1
78
(27.894973754883): SELECT show_live FROM jos_com_show_setting LIMIT 1
79
(61.03515625): SELECT show_live FROM jos_com_show_setting LIMIT 1
80
(45.061111450195): SELECT show_live FROM jos_com_show_setting LIMIT 1
81
(59.127807617188): SELECT show_live FROM jos_com_show_setting LIMIT 1
82
(37.908554077148): SELECT show_live FROM jos_com_show_setting LIMIT 1
83
(!!!!! 1297.9507446289): SELECT show_live FROM jos_com_show_setting LIMIT 1
84
(87.02278137207): SELECT show_live FROM jos_com_show_setting LIMIT 1
85
(41.961669921875): SELECT show_live FROM jos_com_show_setting LIMIT 1
86
(30.040740966797): SELECT show_live FROM jos_com_show_setting LIMIT 1
87
(56.028366088867): SELECT fid FROM jos_deeppockets
								WHERE cat=10
								AND id=294779078
								AND (type='deeppockets_item' OR type='deeppockets_item_link') 
								AND published='1' 
								LIMIT 1
88
(30.994415283203): SELECT fid FROM jos_deeppockets_archive WHERE cat=10 AND id=294779078 AND (type='deeppockets_item' OR type='deeppockets_item_link') AND published='1' LIMIT 1
89
(45.061111450195): SELECT id,name,title
 FROM jos_deeppockets WHERE cat=10 AND TYPE='file_folder' AND published=1
			AND id != 294743300
			AND id != 294743301
			AND id != 294743302
			AND id != 294743303
			AND id != 294743304
			AND id != 294743305
			AND id != 294743306
			AND id != 294743307
			AND id != 294743308
			AND id != 294743309
			AND id != 294743310
			AND metakey != 'aacc'
		ORDER BY `ordering`
90
(65.088272094727): SELECT show_live FROM jos_com_show_setting LIMIT 1
91
(35.04753112793): SELECT show_live FROM jos_com_show_setting LIMIT 1
92
(31.948089599609): SELECT show_live FROM jos_com_show_setting LIMIT 1
93
(30.040740966797): SELECT show_live FROM jos_com_show_setting LIMIT 1
94
(28.848648071289): SELECT show_live FROM jos_com_show_setting LIMIT 1
95
(30.040740966797): SELECT show_live FROM jos_com_show_setting LIMIT 1
96
(92.983245849609): SELECT show_live FROM jos_com_show_setting LIMIT 1
97
(31.948089599609): SELECT show_live FROM jos_com_show_setting LIMIT 1
98
(29.087066650391): SELECT show_live FROM jos_com_show_setting LIMIT 1
99
(29.087066650391): SELECT show_live FROM jos_com_show_setting LIMIT 1
100
(37.193298339844): SELECT show_live FROM jos_com_show_setting LIMIT 1
101
(43.153762817383): SELECT show_live FROM jos_com_show_setting LIMIT 1
102
(58.174133300781): SELECT show_live FROM jos_com_show_setting LIMIT 1
103
(34.093856811523): SELECT show_live FROM jos_com_show_setting LIMIT 1
104
(90.122222900391): SELECT * FROM jos_deeppockets WHERE 1=1 AND id=294779078 AND published='1'
				union
		SELECT * FROM jos_deeppockets_archive WHERE 1=1 AND id=294779078 AND published='1'
		LIMIT 1
105
(68.902969360352): SELECT * FROM jos_deeppockets WHERE 1=1 AND id=294779078 AND published='1'
				union
		SELECT * FROM jos_deeppockets_archive WHERE 1=1 AND id=294779078 AND published='1'
		LIMIT 1
106
(82.015991210938): SELECT dp.*, ch.title as channel, exp.name as author_name, exp.specialization as author_specialization, exp.images as author_photo FROM jos_deeppockets as dp
							LEFT JOIN jos_experts as exp
							ON exp.id = dp.author
							LEFT JOIN jos_deeppockets as ch
							ON dp.fid = ch.id
							WHERE 1=1
							 AND dp.cat=10 
							AND dp.id=294779078
							AND (dp.type='deeppockets_item' OR dp.type='deeppockets_item_link')
							AND dp.published='1' LIMIT 1
107
(50.067901611328): SELECT dp.*, ch.title as channel, exp.name as author_name, exp.specialization as author_specialization, exp.images as author_photo FROM jos_deeppockets_archive as dp
								LEFT JOIN jos_experts as exp
								ON exp.id = dp.author
								LEFT JOIN jos_deeppockets as ch
								ON dp.fid = ch.id
								WHERE 1=1
								 AND dp.cat=10 
								AND dp.id=294779078
								AND (dp.type='deeppockets_item' OR dp.type='deeppockets_item_link')
								AND dp.published='1' LIMIT 1
108
(538.11073303223): SELECT * FROM jos_deeppockets WHERE 1=1 AND id=294779078 AND published='1'
				union
		SELECT * FROM jos_deeppockets_archive WHERE 1=1 AND id=294779078 AND published='1'
		LIMIT 1
109
(107.0499420166): SELECT * FROM jos_deeppockets WHERE 1=1 AND id=294779078 AND published='1'
				union
		SELECT * FROM jos_deeppockets_archive WHERE 1=1 AND id=294779078 AND published='1'
		LIMIT 1
110
(77.009201049805): SELECT dp.*, ch.title as channel, exp.name as author_name, exp.specialization as author_specialization, exp.images as author_photo FROM jos_deeppockets as dp
							LEFT JOIN jos_experts as exp
							ON exp.id = dp.author
							LEFT JOIN jos_deeppockets as ch
							ON dp.fid = ch.id
							WHERE 1=1
							
							AND dp.id=294779078
							AND (dp.type='deeppockets_item' OR dp.type='deeppockets_item_link')
							AND dp.published='1' LIMIT 1
111
(502.8247833252): SELECT dp.*, ch.title as channel, exp.name as author_name, exp.specialization as author_specialization, exp.images as author_photo FROM jos_deeppockets_archive as dp
								LEFT JOIN jos_experts as exp
								ON exp.id = dp.author
								LEFT JOIN jos_deeppockets as ch
								ON dp.fid = ch.id
								WHERE 1=1
								
								AND dp.id=294779078
								AND (dp.type='deeppockets_item' OR dp.type='deeppockets_item_link')
								AND dp.published='1' LIMIT 1
112
(598.90747070312): SELECT dp.name, dp.id, dp2.name as channel, ex.name as author_name, ex.specialization, ex.images FROM jos_deeppockets as dp
							LEFT JOIN jos_experts as ex
							ON ex.id = dp.author
							LEFT JOIN jos_deeppockets as dp2
							ON dp2.id = dp.fid
							WHERE dp.author != 0
							AND dp.cat = 10
							AND dp.published = 1
							AND (
								(dp.fid = 294743300)
								OR (dp.fid = 294743301)
								OR (dp.fid = 294743302)
								OR (dp.fid = 294743303)
								OR (dp.fid = 294743304)
								OR (dp.fid = 294743305)
								OR (dp.fid = 294743306)
								OR (dp.fid = 294743307)
								OR (dp.fid = 294743308)
								OR (dp.fid = 294743309)
								OR (dp.fid = 294743310)
								)
							ORDER BY RAND()
							LIMIT 1
113
(448.94218444824): SELECT dp.name, dp.id, dp2.name as channel, ex.name as author_name, ex.specialization, ex.images FROM jos_deeppockets as dp
							LEFT JOIN jos_experts as ex
							ON ex.id = dp.author
							LEFT JOIN jos_deeppockets as dp2
							ON dp2.id = dp.fid
							WHERE dp.author != 0
							AND dp.cat = 10
							AND dp.published = 1
							AND (
								(dp.fid = 294743300)
								OR (dp.fid = 294743301)
								OR (dp.fid = 294743302)
								OR (dp.fid = 294743303)
								OR (dp.fid = 294743304)
								OR (dp.fid = 294743305)
								OR (dp.fid = 294743306)
								OR (dp.fid = 294743307)
								OR (dp.fid = 294743308)
								OR (dp.fid = 294743309)
								OR (dp.fid = 294743310)
								)
							ORDER BY RAND()
							LIMIT 1
114
(77.962875366211): SELECT * FROM jos_deeppockets WHERE 1=1 AND id=294779078 AND published='1'
				union
		SELECT * FROM jos_deeppockets_archive WHERE 1=1 AND id=294779078 AND published='1'
		LIMIT 1
115
(66.041946411133): SELECT * FROM jos_deeppockets WHERE 1=1 AND id=294779078 AND published='1'
				union
		SELECT * FROM jos_deeppockets_archive WHERE 1=1 AND id=294779078 AND published='1'
		LIMIT 1
116
(118.97087097168): SELECT * FROM jos_deeppockets WHERE 1=1 AND id=294779078 AND published='1'
				union
		SELECT * FROM jos_deeppockets_archive WHERE 1=1 AND id=294779078 AND published='1'
		LIMIT 1
117
(51.021575927734): SELECT * FROM jos_deeppockets WHERE 1=1 AND id=294779078 AND published='1'
				union
		SELECT * FROM jos_deeppockets_archive WHERE 1=1 AND id=294779078 AND published='1'
		LIMIT 1
118
(78.916549682617): SELECT * FROM jos_deeppockets WHERE 1=1 AND id=294779078 AND published='1'
				union
		SELECT * FROM jos_deeppockets_archive WHERE 1=1 AND id=294779078 AND published='1'
		LIMIT 1
119
(41.961669921875): SELECT * FROM jos_deeppockets WHERE 1=1 AND id=294779078 AND published='1'
				union
		SELECT * FROM jos_deeppockets_archive WHERE 1=1 AND id=294779078 AND published='1'
		LIMIT 1
120
(302.07633972168): SELECT * FROM trending_articles WHERE `journal_id` = 10 AND `timestamp` <= NOW() + INTERVAL 7 DAY ORDER BY `order`
121
(!!!!! 92444.896697998): SELECT
				*
			FROM
				jos_deeppockets
			WHERE
				jos_deeppockets.cat = 10
			AND
				jos_deeppockets.type = 'deeppockets_item'
			AND
				jos_deeppockets.published = 1
			AND
				jos_deeppockets.created + INTERVAL 14 DAY >= NOW()
			ORDER BY
				jos_deeppockets.hits DESC
			LIMIT
				7
122
(101.08947753906): SELECT * FROM jos_deeppockets WHERE 1=1 AND id=294779078 AND published='1'
				union
		SELECT * FROM jos_deeppockets_archive WHERE 1=1 AND id=294779078 AND published='1'
		LIMIT 1
123
(45.061111450195): SELECT * FROM jos_deeppockets WHERE 1=1 AND id=294779078 AND published='1'
				union
		SELECT * FROM jos_deeppockets_archive WHERE 1=1 AND id=294779078 AND published='1'
		LIMIT 1
124
(447.98851013184): SELECT fid FROM jos_deeppockets WHERE cat=10 AND id=294779078 AND type='deeppockets_item' AND published='1' union SELECT fid FROM jos_deeppockets_archive WHERE cat=10 AND id=294779078 AND type='deeppockets_item' AND published='1'  LIMIT 1
125
(!!!!! 672547.10197449): (
				SELECT
					dp.id,
					dp.name,
					dp.title,
					dp.video,
					dp.video_source,
					dp.created

				FROM
					jos_deeppockets AS dp

				WHERE
					dp.video != ''
				AND
					dp.video_source != ''
				AND
					dp.cat = 10
				AND
					dp.published = 1
				AND
					dp.type != 'deeppockets_item_link'

				

				ORDER BY
					created DESC

				LIMIT
					3
			)
			UNION
			(
				SELECT
					dpa.id,
					dpa.name,
					dpa.title,
					dpa.video,
					dpa.video_source,
					dpa.created

				FROM
					jos_deeppockets_archive AS dpa

				WHERE
					dpa.video != ''
				AND
					dpa.video_source != ''
				AND
					dpa.cat = 10
				AND
					dpa.published = 1
				AND
					dpa.type != 'deeppockets_item_link'

				

				ORDER BY
					created DESC

				LIMIT
					3
			)
			ORDER BY
				created DESC

			LIMIT
				3
126
(74.148178100586): (
				SELECT
					dp.id,
					dp.name,
					dp.title,
					dp.video,
					dp.video_source,
					dp.created

				FROM
					jos_deeppockets AS dp

				WHERE
					dp.video != ''
				AND
					dp.video_source != ''
				AND
					dp.cat = 10
				AND
					dp.published = 1
				AND
					dp.type != 'deeppockets_item_link'

				

				ORDER BY
					created DESC

				LIMIT
					3
			)
			UNION
			(
				SELECT
					dpa.id,
					dpa.name,
					dpa.title,
					dpa.video,
					dpa.video_source,
					dpa.created

				FROM
					jos_deeppockets_archive AS dpa

				WHERE
					dpa.video != ''
				AND
					dpa.video_source != ''
				AND
					dpa.cat = 10
				AND
					dpa.published = 1
				AND
					dpa.type != 'deeppockets_item_link'

				

				ORDER BY
					created DESC

				LIMIT
					3
			)
			ORDER BY
				created DESC

			LIMIT
				3
127
(46.968460083008): SELECT
				dp.id,
				dp.name,
				dp.title,
				dp.video,
				dp.video_source,
				dp.created

			FROM
				jos_deeppockets AS dp

			WHERE
				dp.id = 294800513

			LIMIT 1
128
(29.087066650391): SELECT
				dp.id,
				dp.name,
				dp.title,
				dp.video,
				dp.video_source,
				dp.created

			FROM
				jos_deeppockets AS dp

			WHERE
				dp.id = 294800513

			LIMIT 1
129
(41.007995605469): SELECT
				dp.id,
				dp.name,
				dp.title,
				dp.video,
				dp.video_source,
				dp.created

			FROM
				jos_deeppockets AS dp

			WHERE
				dp.id = 294800359

			LIMIT 1
130
(30.040740966797): SELECT
				dp.id,
				dp.name,
				dp.title,
				dp.video,
				dp.video_source,
				dp.created

			FROM
				jos_deeppockets AS dp

			WHERE
				dp.id = 294800359

			LIMIT 1
131
(29.087066650391): SELECT
				dp.id,
				dp.name,
				dp.title,
				dp.video,
				dp.video_source,
				dp.created

			FROM
				jos_deeppockets AS dp

			WHERE
				dp.id = 294800361

			LIMIT 1
132
(28.133392333984): SELECT
				dp.id,
				dp.name,
				dp.title,
				dp.video,
				dp.video_source,
				dp.created

			FROM
				jos_deeppockets AS dp

			WHERE
				dp.id = 294800361

			LIMIT 1
133
(537.87231445312): SELECT *
                            FROM jos_deeppockets
                            WHERE 1=1 
                              AND id=294779078
                              AND (type='deeppockets_item' OR type='deeppockets_item_link')
                              AND published='1'
                            LIMIT 1
134
(404.83474731445): SELECT *
                            FROM jos_deeppockets_archive
                            WHERE 1=1 
                              AND id=294779078
                              AND (type='deeppockets_item' OR type='deeppockets_item_link')
                              AND published='1'
                            LIMIT 1
135
(39.100646972656): SELECT name FROM jos_deeppockets WHERE id=215
136
(31.948089599609): SELECT name FROM jos_deeppockets WHERE id=215
137
(471.83036804199): SELECT *,
    DATE_FORMAT(publish_up,'%d %b %Y') AS date_publish_up,
    DATE_FORMAT(publish_down,'%d %b %Y') AS date_publish_down
    FROM jos_events
    WHERE state=1
    AND publish_up > NOW()
    and extra_info REGEXP '((;)|(^))215((;)|($))' AND catid IN (0,110) 
    ORDER BY publish_up ASC LIMIT 3
138
(353.81317138672): SELECT *,
    DATE_FORMAT(publish_up,'%d %b %Y') AS date_publish_up,
    DATE_FORMAT(publish_down,'%d %b %Y') AS date_publish_down
    FROM jos_events
    WHERE state=1
    AND publish_up > NOW()
    and extra_info REGEXP '((;)|(^))215((;)|($))' AND catid IN (0,110) 
    ORDER BY publish_up ASC LIMIT 3
139
(59.127807617188): SELECT * FROM jos_deeppockets WHERE 1=1 AND id=294779078 AND published='1'
				union
		SELECT * FROM jos_deeppockets_archive WHERE 1=1 AND id=294779078 AND published='1'
		LIMIT 1
140
(66.995620727539): SELECT * FROM jos_deeppockets WHERE 1=1 AND id=294779078 AND published='1'
				union
		SELECT * FROM jos_deeppockets_archive WHERE 1=1 AND id=294779078 AND published='1'
		LIMIT 1
141
(!!!!! 8379.9362182617): TRUNCATE TABLE jos_trademed_module
142
(93.936920166016): SELECT dp.*, ch.title as channel, exp.name as author_name, exp.specialization as author_specialization, exp.images as author_photo FROM jos_deeppockets as dp
							LEFT JOIN jos_experts as exp
							ON exp.id = dp.author
							LEFT JOIN jos_deeppockets as ch
							ON dp.fid = ch.id
							WHERE 1=1
							 AND dp.cat=10 
							AND dp.id=294779078
							AND (dp.type='deeppockets_item' OR dp.type='deeppockets_item_link')
							AND dp.published='1' LIMIT 1
143
(61.03515625): SELECT dp.*, ch.title as channel, exp.name as author_name, exp.specialization as author_specialization, exp.images as author_photo FROM jos_deeppockets_archive as dp
								LEFT JOIN jos_experts as exp
								ON exp.id = dp.author
								LEFT JOIN jos_deeppockets as ch
								ON dp.fid = ch.id
								WHERE 1=1
								 AND dp.cat=10 
								AND dp.id=294779078
								AND (dp.type='deeppockets_item' OR dp.type='deeppockets_item_link')
								AND dp.published='1' LIMIT 1
144
(792.9801940918): SELECT *
   FROM jos_deeppockets
   WHERE 1=1 
   AND id=294779078
   AND (type='deeppockets_item' OR type='deeppockets_item_link') AND published='1' LIMIT 1
145
(97.036361694336): SELECT *
   FROM jos_deeppockets
   WHERE 1=1 
   AND id=294779078
   AND (type='deeppockets_item' OR type='deeppockets_item_link') AND published='1' LIMIT 1
146
(53.882598876953): SELECT * FROM jos_deeppockets WHERE 1=1 AND id=294779078 AND published='1'
				union
		SELECT * FROM jos_deeppockets_archive WHERE 1=1 AND id=294779078 AND published='1'
		LIMIT 1
147
(41.007995605469): SELECT * FROM jos_deeppockets WHERE 1=1 AND id=294779078 AND published='1'
				union
		SELECT * FROM jos_deeppockets_archive WHERE 1=1 AND id=294779078 AND published='1'
		LIMIT 1
148
(!!!!! 1163.0058288574): SELECT jos_item.*
						FROM jos_item INNER JOIN jos_number ON jos_item.numberID=jos_number.id
						WHERE 1=1
						AND (jos_item.imgMini<>'')
						AND jos_item.type='L'
						AND jos_item.numberID= (
									 SELECT jos_number.id
									 FROM jos_number
									 WHERE jos_number.journalID IN (10)
						
									and jos_number.id in (select distinct jos_number.id from jos_number join jos_item on jos_item.numberid=jos_number.id where jos_item.type='P')
									 ORDER BY (CONVERT(SUBSTRING(issue,1,2),SIGNED) + (CONVERT(SUBSTRING(issue,3,2),SIGNED)*100)) DESC
									 LIMIT 0,1
									 )
						ORDER BY RAND() DESC LIMIT 6
149
(72.956085205078): SELECT * FROM jos_deeppockets WHERE 1=1 AND id=294779078 AND published='1'
				union
		SELECT * FROM jos_deeppockets_archive WHERE 1=1 AND id=294779078 AND published='1'
		LIMIT 1
150
(43.869018554688): SELECT * FROM jos_deeppockets WHERE 1=1 AND id=294779078 AND published='1'
				union
		SELECT * FROM jos_deeppockets_archive WHERE 1=1 AND id=294779078 AND published='1'
		LIMIT 1
151
(33.140182495117): SELECT fid FROM jos_deeppockets WHERE cat=10 AND id=294779078 AND type='deeppockets_item' AND published='1' union SELECT fid FROM jos_deeppockets_archive WHERE cat=10 AND id=294779078 AND type='deeppockets_item' AND published='1'  LIMIT 1
152
(29.087066650391): select distinct links.*
from jos_textlinks links
join jos_textlinks_placement placement on links.id=placement.link_id
where journal_id=(10) and (channel_id=215 or channel_id is null)
153
(26.941299438477): select distinct links.*
from jos_textlinks links
join jos_textlinks_placement placement on links.id=placement.link_id
where journal_id=(10) and (channel_id=215 or channel_id is null)
154
(34.093856811523): SELECT livesite FROM jos_dp_categories WHERE id=10 AND published='1' LIMIT 1
155
(62.942504882812): SELECT * FROM jos_deeppockets WHERE 1=1 AND id=294779078 AND published='1'
				union
		SELECT * FROM jos_deeppockets_archive WHERE 1=1 AND id=294779078 AND published='1'
		LIMIT 1
156
(45.061111450195): SELECT * FROM jos_deeppockets WHERE 1=1 AND id=294779078 AND published='1'
				union
		SELECT * FROM jos_deeppockets_archive WHERE 1=1 AND id=294779078 AND published='1'
		LIMIT 1
157
(41.007995605469): select jos_twitters.link from jos_twitters where cat=10
158
(31.948089599609): select jos_facebook.link from jos_facebook where cat=10
159
(32.186508178711): select jos_google_plus.link from jos_google_plus where cat=10
160
(36.001205444336): select jos_linkedin.link from jos_linkedin where cat=10
161
(243.9022064209): update jos_bannersmanager_banner
			set impmade=impmade+1
			where id in(14615,14202,14203)
162
(!!!!! 1301.0501861572): insert into jos_banners_delta values (14615,'2025-06-19 11:54:57','216.73.216.120','view'),(14202,'2025-06-19 11:54:57','216.73.216.120','view'),(14203,'2025-06-19 11:54:57','216.73.216.120','view')